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Can a long gene really go from four fragments to a full-length clone in just 15 days?
In a recent 8 kb gene synthesis case,
Synbio Technologies
assembled four DNA fragments (A/B/C/D) directly with a linearized vector in a single in vivo recombination step.
Case Highlights
•
8 kb target gene
•
4 DNA fragments assembled simultaneously
•
One-step
in vivo
recombination
•
15 days from correct fragments to full-length correct clone
•
Sequence features included low-GC regions and small hairpin structures
Why One-Step Assembly Matters
Traditional stepwise assembly may require three separate transformation and screening cycles, with a theoretical timeline of around 20 days before the first full-length clone.
Our approach helps reduce:
•
Multiple assembly and transformation cycles
•
Cumulative DNA loss and handling
•
Intermediate construct screening
•
Time associated with sequential assembly
From sequence optimization and fragment synthesis to assembly, clone screening, and verification, we provide an integrated workflow for challenging
long-gene projects
.
Working on a long or challenging gene construct?
Discuss your project with our team
Looking for more? These services might be just what you need:
DNA Synthesis
RNA Synthesis
Protein Expression
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Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA